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    • 专利摘要:
    1440642 Reducing RNA content of microfungi RANKS HOVIS MCDOUGALL Ltd 23 Sept 1974 [24 Sept 1973] 44708/73 Heading C6F A process for reducing the nucleic acid content of an edible protein containing substance involves maintaining cells of a grown non-toxic strain of the genus Fusarium in a suspension at a pH of between 4À7 and 7À0 and at a temperature between 55 and 72‹C for a time of at least 60 seconds, typically for 5 to 60 minutes. The microfungus may initially have been held at an elevated pH for sufficient time to destroy the protease of the cells but not the ribonuclease typically at a pH of 8À5 and at 65 C for between 1/2 minute and 5 minutes. Microfungi: Fusarium graminearum Schwabe IMI 145425, Fusarium oxysporum IMI 154214 and Fusarium solani IMI 154217. The resulting fungal mycelium possesses a reduced level of RNA of below 4 wt. per cent. Comparative experiments disclose the treatment of the microfungus at pH's and temperatures outside the defined ranges.
    公开号:SU1082304A3
    申请号:SU742067115
    申请日:1974-09-23
    公开日:1984-03-23
    发明作者:Джон Товерсей Питер；Лонгтон Джон；Норман Кокрэм Джеффри
    申请人:Рэнкс Ховис Макдугалл Лимитед (Фирма)；
    IPC主号:
    专利说明:

    The invention relates to a technique for producing edible products containing edible food protein from non-toxic microscopic fungi, and can be used in the microbiological industry.
    A method of obtaining protein food products is known by preparing a suspension of non-toxic microscopic fungi and exposing it when heated at a certain pH value l {,
    The disadvantage of this method is the increased content of nucleic acids in the product, equal to 7-12%, which is unacceptable when it is used as a food product, since the content of nucleic acid in the product must be at a level that allows the absorption of nucleino. howl acid no more than 2 g per day, t. e not more than 4 weight,%, In addition, an increased loss of protein during the processing of the microorganism
    The aim of the invention is to reduce the content of nucleic acids in the products, as well as to reduce the loss of protein.
    This goal is achieved in that according to the method of producing protein food products, the suspension is prepared from non-toxic strains Fusarium graminearum Schwabe JMJ 145425 or Fusarium oxyeporum JMJ 154214, or Fusarim SoSani JMJ 154217, and the suspension is carried out at pH 4.7-7.0, temperature 55-72 0 for 1-60 minutes.
    The method is carried out as follows.
    The grown microbial protein or fungal mycelium of non-toxic microorganisms of Fusarium graminearum Schwabe JMJ 145425 or Fusarium oxysporum JMJ 154214, or Fusarium SoEani JMJ 154217 strains is filtered, washed out and washed. Then the cells of microorganisms are mixed with a buffer solution with a pH of 4.7-7.0 and maintained at this pH value and heated at 55-72 s for 60-200 s. The cells can then be suspended again and incubated in tap water at pH 6.3 at 63 ° C for 20 minutes.
    The resulting mycelium can contain ribonucleic acid (RNA) 1–4% compared to 7–12% of the untreated organism. In some cases, the RNA content may be less than 1%. The RNA content is determined by the modified Schneider method, total nitrogen (TH) - in the automatic instrument Kjeldal, and amine nitrogen (AN) - according to the modified Pinnegar method,
    Example 1. Fusariun graminea rum Schwabe 145425 cultured continuously on medium in tap water containing the following ingredients, g / l:
    Potato starch (processed with oC-amylase and glukamylazoy) 60
    MgS047H200.75
    ZnSO 7H2010.0
    CuSO45H202,0
    PUMP 2P-202,0
    Sose. , 0
    MnSO4-4H2O10.0
    NH4H P043.0
    Kj, 4
    Nace0,125
    and ingredients, mg / l:
     7H2O 20, O
    Zornic acid 0.5
    Bioty0.005
    PPG 20000.04
    Growth conditions: temperature 30 ° C, pH 6.0, pressure 1.09 kg / cm, agitator speed 230 rpm, air supply 800 l / min, dissolved oxygen 6.5 (manufactured by air increase 80), the dilution rate is 0.14 h, the sterilization temperature is 135 ° C (continuous sterilization at a shutter speed of 90 s), the fermentation tank volume is 1300 l.
    Before continuous cultivation, the tank works with 20 liters of seed of the cultivated crop, and after the growth is completed, I conduct the process in the tank continuously,
    In the following examples, the RNA content is not adjusted for biomass loss,
    The RNA content (not corrected) in biomass loss is 2%, i.e. 2 g per 100 g of the original cells, while the percentage of (corrected) in biomass loss is 2.86%, m, e, 2 g in final 70 g of treated cells.
    Conducting an isothermal process leads to a loss of biomass of about 30%, but since it is not determined on every sample, the content of RNA is calculated as a function of the starting material.
    EXAMPLE 2 Fusarium graminearum Schwabe 145425 is cultured as described in Example 1. The cells are collected, filtered and washed according to the Büchner method, and then suspended in tap water in an amount of 10 g / at different temperatures and times of incubation.
    The test results are shown in Table 1.
    Table
    Continued table. one
    Continued table. one
    Thus, the data table. 1 show that the level of nucleic acid is reduced to acceptable values in the temperature range of 55-72 ° C.
    The ideal isothermal temperature depends on the degree of PIDC removal required and the duration of treatment allowed for economic reasons.
    Preferred conditions are: pH 6, temperature 62.5 ° C for 18 minutes.
    P D and measure 3. Nucleic acid reduction efficiency with pi; 4 - 9.5 and 62.5 ° C.
    Fusarium qraminearum Schwabe JMJ 145425 is cultivated as described in Example 1- harvested and filtered by washing using the Buchner method. Zateg
    cells are suspended in tap water in an amount of 10 g / l, ipH is half-supported at a given level by automatic addition of HCP or NHijCe. Samples are incubated with 18 mi: n. The test results are given in t abl. 2
    Table 2
    Thus, the data table. 2 show that pH. for the process at 62f5 ° C is in the range of 4.7-7.0; the maximum decrease in RNA is observed at pH 6. The optimum pH for preserving the protein is 8.5-9.0.
    The dry solids of wet filter cake and suspension at pH 6.0 and above is dark gray, at pH 5.0 it is yellowish brown and at pH 4.0 it is white. .This way at the end of the process is desirable
    In the studied limit of NASI and 0, there was no effect on the process of reducing RNA.
    Example 5, Reduction efficiency of nucleic acid at various concentrations of suspensions. Strain Fusarium graminearum Schwabe JMJ
    adjust pH to 4.0 to give a white product.
    Example 4, Efficacy of reducing nucleic acid in solution -. pax with different ionic strength. The process is carried out with the strain
    Eusarium graminearum Schwabe JMJ 145425 as described in example 3 at pH 6.0 and 62.5 ° C for 20 min. S. by adding NaC-C, in the amount of 0.01-0.50 M and NHijC - 0.5 M in distilled water,
    Test data are given in table. 3
    Table3
    145425, cultured as previously described, collected, filtered and washed according to the Büchner method, then the cells were suspended in tap water at varying amounts and at different exposures.
    Research data are summarized in Table 4.
    T a
    persons 4
    8.85 7.46 3.27 2.37
    1.85
    The data table. 4 shows that with a high concentration of suspension, stirring is required to quickly equalize the temperatures.
    Example 6 Nucleic Acid Reduction Effectiveness Under Different Mixing Conditions. The strain Fusarium graminearum Schwahe JMJ 145425 cultiviru.ot as described previously. The cells are harvested, filtered and washed according to the Züchner method and suspended in tap water under various mixing conditions.
    The test results show that there is no need to rewind the slurry during the isothermal process. The effect of agitation is insignificant, but entails enormous difficulties from the chemical-technological side, since it requires an increase in size to a large installation.
    Example. Typical nucleic acid reduction experience. Strain
    Continued table. four
    Fusarium graminearum Schwabe JMJ 145425 is cultured as previously described. The cells are collected, filtered and washed according to the Buchner method and suspended in a solution of 0.1 M NaCf in an amount of 10 g / l and incubated at pH 6.0 and 62 for 20 mi
    The product contains 44% protein and 0.6% RNA, i.e. the ratio of protein to HyKj; eHHOBofl acid is 55: 1. The starting material contains 37.7% of protein and 8.5% of RNA with a protein to nucleic acid ratio of 4.35: 1.
    Example Destruction of protease activity without destruction of ribonucleic activity.
    The strain of Fusarium graminearum Schwabe JMJ 145425 cultured as described previously, collected, filtered and washed according to the method of Vyuhner, Cells suspended in distilled water at a. the amount of 10 g / l.
    The pH of the substrate is 8.5 at various times.
    Then, from the cells, the HF is removed with an isothermal process at pH 6 for 20 min;
    Thus, at maximum time, the maximum amount of RJ g is removed, the maximum amount of biomass and amine nitrogen remains in the process of suspension and subsequent isothermal process.
    PRI me R 9, Strain Fusarium
    soВ ani JMJ 154217 cultivated
    following way.
    Cultivation medium contains
    in distilled water the following
    ingredients g / l1
    MgSOiVH ~ 0.25.
    КН2.РО415 f О
    (NH)), SO42.83
    NaOH1,0
    : and ingredients, mg / l:
    Birtin0.05
    Choline50
    Hydroelements5
    Glucose (10% solution after sterilization of 20 ml of trace elements basic solution
    Example 10 o Strain Fusarj.um ozguzrogit JML 154214 is cultivated as described in Example 9 with the exception that the medium contains 0.5 g / l of yeast oxide extract and 0.5 g / l of school peptone in addition with gIjO
    MPSE2, 4H2O1.0
     , 0
     ai-jgO, 0,2
      , 2
    CoSEG- 6bgO0,2
    CaCgj-, 0
    All components except glucose are sterilized together. The material was dissolved in the amount required for 1 l of medium, made up to 850 ml and distributed into five 1 l conical flasks, each containing 170 ml.
    A 10% w / v glucose solution is prepared and sterilized in flasks in portions of 20 MP in an autoclave under a pressure of 1.05 kg / cm for 15 minutes. Before sowing 10 l of the spore suspension, the sterile contents of one glucose flask are added to each flask, cultivated with gushing at 160 rpm and 30 ° C.
    The culture is harvested after 48 hours. The cells are then harvested, filtered by washing using the Züchner method, and suspended in an amount of 10 g / l in tap water at 6 4 ° C at pH 6, set using NaOH or HgSO,
     The data of is-1 data are given in Table 5.
    Table5
    to the composition of the medium described in example 9.
    The cells were collected after 72 hours and the nucleic acid reduction process was carried out as in Example 9.
    These tests are given in Table 6.
    T and b l and d and 6 Results tab. b show that by the processing described, the level of nucleic acid can be effectively reduced. Example 11. The reduction of nucleic acid in the pilot plant. The Fusarium strain, graminearum Schwa be JMJ 145425, is cultured as previously described and treated without separation from the growth medium as follows. A suspension of the mycelium with a strain of .20 g / l, which is not in the fermentation tea at pH 6, is fed to a mono pump. The slurry is pumped into the steam injector, while its temperature quickly rises from 30 to. at. for 5 s, and served on
    Dry Untreated8,22
    Dry material
    with a decrease in RN0.43 The proposed method of obtaining a food additive provides the possibility of obtaining a fungal mycelium having an RNA level of less than 4%, 20
    Tables a. 7
    8.74
    6.45 8.30 6.86 pipeline while maintaining its temperature for 45 minutes The suspension is then passed through a heat exchanger to cool to 20c in order to reduce the possibility of subsequent microbial infection. I am fed into a rotary vacuum filter trough. The liquid is sucked off through the filter sheet, and the mycelium is accumulated on the filter, while the drum carrying the mycelial cake, rotates above the liquid level. The filter cake is washed with a double volume of water, filtered to contain 70% moisture, removed from the drum, suspended in water and spray dried. Test data are given in table, 7. c respectfully below 2 wt. %, as well as the minimum loss of protein in the grown microorganism and the purity of the target product from extraneous chemicals.
    权利要求:
    Claims (1)
    [1]
    METHOD FOR PRODUCING PROTEIN FOOD PRODUCTS by preparing a suspension from microscopic non-toxic mushrooms and holding it; when heated at a certain pH value, characterized in that, in order to reduce the content of nucleic acids in the products, as well as to reduce protein loss, the suspension is prepared from non-toxic strains of Fusarium graminearum Schwabe JMJ 145425 or Fusarium oxysporum'JMJ, 154214 or Fusarium sotani JMJ '''154217, and the suspension is aged at a pH of 4.7-7.0, at a temperature of 55-72 ° C for 1-60 minutes. from
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    同族专利:
    公开号 | 公开日
    US4041189A|1977-08-09|
    FI275774A|1975-03-25|
    SE404579B|1978-10-16|
    FI52995C|1978-01-10|
    CA1025271A|1978-01-31|
    JPS5063197A|1975-05-29|
    SE7411827L|1975-03-25|
    BR7407863D0|1975-07-29|
    JPS605278B2|1985-02-09|
    FI52995B|1977-09-30|
    FR2244407A1|1975-04-18|
    DE2445254A1|1975-04-03|
    GB1440642A|1976-06-23|
    DE2445254C2|1987-04-23|
    FR2244407B1|1978-08-11|
    IT1060852B|1982-09-30|
    JPS5851887A|1983-03-26|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US2450055A|1945-01-06|1948-09-28|Friedrich F Nord|Food composition containing fusaria|
    US3937654A|1970-05-14|1976-02-10|Ranks Hovis Mcdougall Limited|Production of edible protein substances|
    US3775393A|1970-12-03|1973-11-27|Standard Oil Co|Ammonia extraction of unicellular microorganisms|
    US3784536A|1971-02-04|1974-01-08|Standard Oil Co|Process for reducing the nucleic acid content of single cell protein affording microorganisms|
    US3809776A|1971-02-22|1974-05-07|Standard Oil Co|Enzymic degradation of nucleic acids in scp materials|GB1498688A|1975-07-16|1978-01-25|Ici Ltd|Treatment of single cell protein|
    DD124534A1|1976-03-01|1977-03-02|
    JPS5625314B2|1976-04-26|1981-06-11|
    CA1077772A|1976-05-21|1980-05-20|Erich Haid|Method of recovering protein of low nucleic acid content from microorganisms|
    US4079048A|1976-09-10|1978-03-14|Standard Oil Company |Process for preparing functional yeast proteins using alkaline conditions|
    US4163692A|1977-04-25|1979-08-07|E. I. Du Pont De Nemours And Company|Low phosphate growth of fungal mycelia|
    US4168262A|1978-03-13|1979-09-18|Cornell Research Foundation, Inc.|Anhydride modified microbial protein having reduced nucleic acid levels|
    US4341802A|1980-10-24|1982-07-27|Provesto Corporation|Production of protein with reduced nucleic acid|
    GB8308162D0|1983-03-24|1983-05-05|Ranks Hovis Mcdougall Plc|Edible protein containing substances|
    JPH02111249A|1989-09-14|1990-04-24|Alps Electric Co Ltd|Positioning and fixing method of substrate for detecting number of revolutions of motor and stator side substrate|
    GB9011347D0|1990-05-21|1990-07-11|Ici Plc|Production of a proteinaceous composition|
    GB9403930D0|1994-03-01|1994-04-20|Zeneca Ltd|Production of food|
    US6060305A|1994-06-30|2000-05-09|Novo Nordisk Biotech, Inc.|Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system|
    GB9525902D0|1995-12-16|1996-02-21|Zeneca Ltd|Fungus|
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    GB2557781B|2016-06-27|2019-10-30|Marlow Foods Ltd|Edible fungus|
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    GB201919079D0|2019-12-20|2020-02-05|3F Bio Ltd|Process and product thereof|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    GB4470873A|GB1440642A|1973-09-24|1973-09-24|Production of edible protein containing substances|
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